Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction.
نویسندگان
چکیده
We present two real-time reverse-transcription polymerase chain reaction assays for a novel human coronavirus (CoV), targeting regions upstream of the E gene (upE) or within open reading frame (ORF)1b, respectively. Sensitivity for upE is 3.4 copies per reaction (95% confidence interval (CI): 2.5–6.9 copies) or 291 copies/mL of sample. No cross-reactivity was observed with coronaviruses OC43, NL63, 229E, SARS-CoV, nor with 92 clinical specimens containing common human respiratory viruses. We recommend using upE for screening and ORF1b for confirmation.
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عنوان ژورنال:
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
دوره 17 39 شماره
صفحات -
تاریخ انتشار 2012